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KMID : 0604520080340030233
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Journal of the Society of Cosmetic Scientists of Korea
2008 Volume.34 No. 3 p.233 ~ p.244
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Antioxidative Activity and Component Analysis of Psidium guajava Leaf Extracts
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Yang Hee-Jung
Kim Eun-Hee
Park Soo-Nam
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Abstract
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In this study, the antioxidative effects, inhibitory effects on elastase and tyrosinase, and component analysis of Psidium guajava leaf extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities of extract/fractions of Psidium guajava leaf were in the order: 50% ethanol extract < ethyl acetate fraction < deglycosylated flavonoid aglycone fraction . Reactive oxygen species (ROS) scavenging activities of some Psidium guajava leaf extracts on ROS generated in system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activities were 50% ethanol extract < ethyl acetate fraction < deglycosylated flavonoid aglycone fraction . Aglycone fraction showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of Psidium guajava leaf on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Psidium guajava leaf extracts suppressed photohemolysis in a concentration dependent manner , particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect . Aglycone fraction obtained from the deglycosylation reaction of ethyl acetate fraction among the Psidium guajava leaf extracts, showed 1 band in TLC and 1 peak in HPLC experiments (360 nm). One component was identified as quercetin. TLC chromatogram of ethyl acetate fraction of Psidium guajava leaf extract revealed 5 bands and HPLC chromatogram showed 5 peaks, which were identified as quercetin 3-O-gentobioside (10.32%) , quercetin 3-O--D-glucoside (isoquercitin, 13.30%), quercetin 3-O--D-galactoside (hyperin, 11.34%), quercetin 3-O--L-arabinoside (guajavarin, 19.70%), quercetin 3-O--L-rhamnoside (quercitrin, 45.33%) in the order of elution time. The inhibitory effect of Psidium guajava leaf extracts on tyrosinase were investigated to assess their whitening efficacy. Finally, their anti-elastase activities were measured to predict the anti-wrinkle efficacy in the human skin. Inhibitory effects on tyrosinase of some Psidium guajava leaf extracts was 50% ethanol extract < ethylacetate fraction < deglycosylated aglycone fraction . Inhibitory effects on elastase of some Psidium guajava leaf extracts was 50% ethanol extract < deglycosylated aglycone fraction < ethylacetate fraction . These results indicate that extract/fractions of Psidium guajava leaf can function as antioxidants in bioloigcal systems, particularly skin exposed to UV radiation by scavenging and other ROS, and protect cellular membranes against ROS. And component analysis of Psidium guajava leaf extract and inhibitory activity on elastase of the aglycone fraction could be applicable to new functional cosmetics for smoothing wrinkles.
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KEYWORD
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Psidium guajava leaf, flavonoid, antioxidative activity, elastase, cosmetics
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